Synchronized Oscillation in the Thalamus

Acad. Sci. U.S.A. 84, 6558 (1987); C. A. Whitlock and 0. N. Witte, ibid. 79, 3608 (1982). 25. A series of 24 18-nucleotide primers with complete identity to the murine Btk cDNA sequence (GenBank L08967) were used to generate 380base pair overlapping products spanning the entire btk coding sequence. Full sequence information is available from the authors. 26. Filter lifts containing 5 x 105 plaques from a 129/Sv murine genomic X phage library were screened with a 0.6-kb unique region murine cDNA probe as described (3). Clones Btk-C and Btk-D were evaluated by restriction mapping, subcloned into pGEM7ZF(+), and partially sequenced by Sequenase (U.S. Biochemical) with T7, SP6, and btk unique region oligonucleotide primers. 27. E. S. Kawasaki, in PCR Protocols: A Guide to Methods and Applications, M. A. Innis et al., Eds. (Academic Press, San Diego, 1990), pp. 21-27. 28. M. A. Innis and D. H. Gelfand, in ibid., pp. 3-12; R. K. Saiki et al., Science 230, 1350 (1985). 29. We thank P. Soriano for the 129/Sv genomic library; J. Thomas, W. Paul, and collaborators for sharing prepublication data; C. Sawyers, S. Smale, and R. Wall for critical reading of the manuscript; and J. Shimaoka and K. Vensel for assistance in manuscript preparation. Supported by National Institutes of Health Immunology Training Grant, the Jonsson Comprehensive Cancer Center, and NIH Grant AR36834 (D.J.R.); Howard Hughes Medical Institute (D.C.S., S.T., L.C., and 0.N.W.) and the Leukemia Society of America (D.C.S.); Schering-Plough (J.C.G., J.F.B., and M.H.); and the National Cancer Institute, Department of Health and Human Services, under contract NO1-CO-74101 with Advanced BioScience Laboratory (D.A.L., N.G.C., and N.A.J.).